0

0

2650 days ago,
859 views

PowerPoint PPT Presentation
Three-Stage Prediction of Protein Beta-Sheets Using Neural Networks, Alignments, and Graph Algorithms Jianlin Cheng and Pierre Baldi Institute for Genomics and Bioinformatics School of Information and Computer Sciences University of California Irvine

Importance of Predicting Beta-Sheet Structure Ab-initio Structure Prediction Fold Recognition Model Refinement Protein Design Protein Folding Coil beta-sheet helix Rendered in Protein Explorer

An Example of Beta-Sheet Architecture Level 1 4 5 2 1 3 6 7 Structure of Protein 1VJG Beta Sheets

An Example of Beta-Sheet Architecture Level 1 Level 2 4 5 Antiparallel 2 1 3 6 7 Parallel Strand Pair Strand Alignment Pairing Direction Structure of Protein 1VJG Beta Sheets

An Example of Beta-Sheet Architecture Level 1 Level 2 Level 3 4 5 Antiparallel H-security 2 1 3 6 7 Parallel Strand Pair Strand Alignment Pairing Direction Structure of Protein 1VJG Beta Sheets Beta Residue Pair

Previous Work Statistical potential approach for strand arrangement (Hubbard, 1994; Zhu and Braun, 1999) Statistical possibilities to enhance beta-sheet auxiliary structure expectation (Asogawa,1997) Information hypothesis approach for strand arrangement (Steward and Thornton, 2000) Neural systems for beta-buildup sets (Baldi, et al., 2000)

Three-Stage Prediction of Beta-Sheets Stage 1 Predict beta-deposit blending probabilities utilizing 2D-Recursive Neural Networks ( 2D-RNN, Baldi and Pollastri, 2003 ) Stage 2 Use beta-buildup matching probabilities to adjust beta-strands Stage 3 Predict beta-strand sets and beta-sheet engineering utilizing chart calculations

Dataset and Statistics Extract proteins with high determination from Protein Data Bank (Berman et al., 2000) Use DSSP (Kabsch and Sander, 1983) to dole out intra-chain beta-sheet structure Use UniqueProt (Mika and Rost, 2003) to lessen excess Use PSI-BLAST (Altschul et al., 1997) to create profiles Statistics

Stage 1: Prediction of Beta-Residue Pairings Using 2D-RNN Target/Output Matrix (m×m) Input Matrix I (m×m) (i,j) 2D-RNN O = f(I) (i,j) T ij : 0/1 O ij : Pairing Prob. I ij X i - 2 X i - 1 X i X i +1 X i +2 X j - 2 X j - 1 X j X j +1 X j +2 |X i – X j | 20 profiles 3 SS 2 SA X i or X j is the position of beta-deposit i or j in the succession

An Example (Target) 1 2 3 4 5 6 7 Protein 1VJG Beta-Residue Pairing Map (Target Matrix)

An Example (Target) 1 2 3 4 5 6 7 Antiparallel Parallel Protein 1VJG Beta-Residue Pairing Map (Target Matrix)

An Example (Prediction)

Stage 2: Beta-Strand Alignment Antiparallel Use yield likelihood grid as scoring network Dynamic programming Disallow holes and utilize the streamlined inquiry calculation Parallel Total number of arrangements = 2(m+n-1)

Strand Alignment and Pairing Matrix The arrangement score is the total of the blending probabilities of the adjusted buildups The best arrangement is the arrangement with the most extreme score Strand Pairing Matrix Strand Pairing Matrix of 1VJG

Stage 3: Prediction of Beta-Strand Pairings and Beta-Sheet Architecture (Constraints) (a) Seven strands of protein 1VJG in grouping request (b) Beta-sheet topology of protein 1VJG

Stage 3: Prediction of Beta-Strand Pairings and Beta-Sheet Architecture (Constraints) (a) Seven strands of protein 1VJG in grouping request 3 accomplices Protein: 1B7G Rendered in Rasmol (b) Beta-sheet topology of protein 1VJG

Minimum Spanning Tree Like Algorithm Strand Pairing Graph (SPG) (a) Complete SPG Strand Pairing Matrix

Minimum Spanning Tree Like Algorithm Strand Pairing Graph (SPG) (b) True Weighted SPG (a) Complete SPG Strand Pairing Matrix Goal : Find an arrangement of associated subgraphs that augment the total of the arrangement scores and fulfill the limitations Algorithm : Minimum Spanning Tree Like Algorithm

An Example of MST Like Algorithm 1 2 3 4 5 6 7 Step 1: Pair strand 4 and 5 1 2 3 4 5 4 5 6 7 Strand Pairing Matrix of 1VJG

An Example of MST Like Algorithm 1 2 3 4 5 6 7 Step 2: Pair strand 1 and 2 1 2 3 4 5 4 5 6 7 2 1 Strand Pairing Matrix of 1VJG N

An Example of MST Like Algorithm 1 2 3 4 5 6 7 Step 3: Pair strand 1 and 3 1 2 3 4 5 4 5 6 7 2 1 3 Strand Pairing Matrix of 1VJG N

An Example of MST Like Algorithm 1 2 3 4 5 6 7 Step 4: Pair strand 3 and 6 1 2 3 4 5 4 5 6 7 2 1 3 6 Strand Pairing Matrix of 1VJG N

An Example of MST Like Algorithm 1 2 3 4 5 6 7 Step 5: Pair strand 6 and 7 1 2 3 4 5 4 5 6 C 7 2 1 3 6 7 Strand Pairing Matrix of 1VJG N

A New Fold Example (Last CASP) True auxiliary structure 1S12 (94 deposits) C EEEEE CCC EEEEE CCCCCHHHHHHHHHHHHHHHHHHHHCCC EEEEEE CC EEEEEE CCCCHHHHHHHHHHHHHHHHHHHHCCCC EEEEE CCCCCC Predicted optional structure by SSpro (Pollastri, et al., 2002) C EEEEEE CC EEEE CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH E HHCCCC EEEE HHHHHHHHHHHHHHHHHHHHHHHHHCCCC EEEEEEE CCC Strand Pairing Matrix Beta Sheet Topology 5 1 2 4 3 1s12 Rendered in Rasmol True: 1-2, 2-4, 3-4, 1-5 Predicted: 1-2, 2-4, 3-4, 4-5

Beta-Residue Pairing Results The precision of arbitrary calculation is 2.3%. ROC Plot

Strand Pairing Results Naïve calculation of blending every single contiguous strand Specificity = 42% Sensitivity = half All strand sets are nearby strand sets. MST like calculation Specificity = 53% Sensitivity = 59% >20% accurately anticipated strand sets are non-nearby strand sets.

Strand Alignment Results On the accurately anticipated strand matches On all local strand combines The precision of blending heading is 15% higher than that of the gauge calculation. The arrangement precision is essentially higher than past techniques.

Future Work and Applications Allow a cycle to handle beta-barrel, permit holes in arrangement for beta lump, include more sources of info (Punta and Rost, 2005) for beta buildup matching forecast Applications Contact outline acknowledgment Ab-initio structure expectation Model refinement Web server and dataset (SCRATCH suite) http://www.ics.uci.edu/~baldig/betasheet.html

Acknowledgment Pierre Baldi, Arlo Randall, Michael Sweredoski NIH give (LM-07443-01) NSF give (EIA-0321390)

SPONSORS

No comments found.

SPONSORS

SPONSORS