SEMI-QUANTITATIVE TEST FOR PARAQUAT IN PLASMA AND URINE Syngenta Crop Protection Basel Switzerland

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Slide 1

SEMI-QUANTITATIVE TEST FOR PARAQUAT IN PLASMA AND URINE Syngenta Crop Protection Basel Switzerland Test created at Syngenta CTL United Kingdom by Bruce Woolen

Slide 2

WHAT SAMPLES ARE AVAILABLE ? Blood plasma is the favored specimen If just blood serum is accessible this can likewise be tried with constraints for translation of results (see slide 19) Urine tests are valuable to affirm that a harming case includes paraquat

Slide 3

DITHIONITE REDUCTION OF Paraquat is changed over to a blue shading by sodium dithionite Sodium dithionite soluble base PARAQUAT RADICAL ION (BLUE)

Slide 4

SUMMARY OF THE TEST PART 1 A pre-screen will identify high centralizations of paraquat in plasma and pee If Part 1 is negative PART 2 A more touchy blue band test will distinguish paraquat down to 0.1 µg/mL

Slide 5

1M WHAT IS NEEDED FOR THE TEST ? 1M NaOH ( FOR URINE) WATER SYNGENTA TEST KIT 15 ML PLASTIC TUBE 5 ML OR 10 ML SYRINGE PLASTIC MICROPIPETTE CEREX SILICA CARTRIDGE GLASS TUBES 1.2 ML SYRINGE ADAPTOR

Slide 6

Sachet B 1g Na 2 S 2 0 4 Sachet A 2g NaHC0 3 PREPARATION OF DITHIONITE REAGENT 2 MINS 10 mL WATER MIX ALLOW TO SETTLE 2 mins

Slide 7

Video cut arrangement of dithionite

Slide 8

PART 1 : PRE-SCREEN IN PLASMA OR URINE X 20 drops approx 0.9 mL MIX PLASMA or URINE DITHIONITE REAGENT COMPARE Color

Slide 9

Color CHART FOR PRE-SCREEN 0 3 10 30 100 µg/mL The breaking point of discovery for paraquat in plasma and pee is around 2-3 µg/mL

Slide 10

Video cut pre-screen

Slide 11

RESULTS OF THE PRE-SCREEN If a blue shading is delivered the convergence of paraquat is evaluated from the graph and no further testing is done If an exceptionally swoon blue shading or no blue shading is created the blue band test is done Urine tests require an extra stride before the blue band test

Slide 12

1M URINE ONLY PREPARATION OF URINE FOR THE BLUE BAND TEST IF TESTING PLASMA PROCEED TO SLIDE NO 14 DISCARD PELLET + URINE 5 mL 1M NaOH 0.5 mL CENTRIFUGE MIX 10 MINS URINE FOR TESTING 3000 RPM

Slide 13

PART 2 : BLUE BAND TEST FOR PARAQUAT IN PLASMA OR URINE DITHIONITE REAGENT approx 1 mL 2 MINS BLUE BAND PLASMA or URINE ~ 3 mL ~ 1 mL WATER COMPARE Color

Slide 14

Color CHART FOR BLUE BAND INTENSITY 0 0.1 0.2 0.5 1 2 µg/mL Chart is appropriate to both plasma and pee

Slide 15

Video clasp of blue band test

Slide 16

Plasma paraquat fixation µg/mL 5 3 a 1.0 0.7 0.5 0.3 b 0.1 0.07 0.05 4 8 2 12 14 6 10 20 22 16 18 24 Time after ingestion (hours) INTERPRETATION OF PLASMA PARAQUAT RESULTS Proudfoot bend – prescient line Examples : a) 1 µg/mL at 8h after ingestion survival far-fetched b) 0.1µg/mL at 12h after ingestion survival likely a survival improbable b survival likely Proudfoot A T, Stewart M S, Levitt T and Widdop B. Paraquat Poisoning : criticalness of plasma paraquat fixations. Lancet 1: 330 1979

Slide 17

INTERPRETATION OF PLASMA PARAQUAT RESULTS From Syngenta paraquat treatment booklet Example : 1 µg/mL at 8h after ingestion roughly 30% shot of survival

Slide 18

WARNING ABOUT SERUM Plasma versus serum Paraquat fixations in plasma and serum are NOT identical (serum focuses are around 3 times lower than plasma focuses) Proudfoot and Syngenta survival bends depend on plasma groupings of paraquat

Slide 19

INTERPRETATION OF URINE PARAQUAT RESULTS From Schermann information Dark blue Navy blue Light blue Pale blue No blue Color Schermann,J.M, Houze P, Bismuth C and Bourdon R. Prognostic estimation of Plasma and pee paraquat fixation. Human Toxicology : 6 91-93 1987

Slide 20

LIST OF EQUIPMENT AND SUPPLIERS Paraquat test unit containing sodium bicarbonate and sodium dithionite (Syngenta) Glass tubes 40 x 6mm 1.2mL (NLG Scientific) Cerex silica cartridges 3mL 20mg Cat no 620-0203 ( Crawford Scientific) Micropipettes Mini-Pastette LW4231 (Alpha Laboratories) Syringe connector 120-1101 (Argonaut Technologies)

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