Mycology Review: Identification of Common Dermatophytes

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Mycology Review: Identification of Common Dermatophytes Sandy Arduin, MT (ASCP) Bruce Palma, MT (ASCP) Mycology Unit Bureau of Laboratories Michigan Department of Community Health This venture was bolstered partially by Grant/Cooperative Agreement NumberU10/CCU523395-01 from the Centers for Disease Control and Prevention (CDC) Its substance are exclusively the obligation of Michigan Department of Community Health and don't really speak to the official perspectives of CDC

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Dermatophytes

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Index Microsporum nanum Differentiation Table Test Your Knowledge Unknown 1 Unknown 2 Unknown 3 Unknown 4 Unknown 5 Unknown 6 Trichophyton rubrum Trichophyton mentagrophytes Hair Perforation Test Trichophyton tonsurans Trichophyton verrucosum Trichophyton terrestre Epidermophyton floccosum Microsporum canis Microsporum gypseum Double tap on any of the words recorded above to go straightforwardly to the slide on that creature. To come back to the record, tap on the home catch. To come back to the keep going slide saw, tap on the arrival catch. You should be in slide indicate mode to utilize these capacities. Naturally visible province morphology portrayals depend on societies developed on SAB agar. Province morphology may change on other culture media.

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Trichophyton rubrum Colony development is ease back to direct, fleece, white at first glance with a red to chestnut invert. Microconidia are club-molded to pyriform and are shaped at the edges of the hyphae. Macroconidia are pencil-formed to stogie molded. Lab tests: hair aperture test negative, urease negative, development at 37 °C. Disease is commonly found on the feet, hands, nails, or crotch.

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Trichophyton mentagrophytes Colony development is tolerably fast, fine to granular, white to cream hued at first glance with a yellowish, chestnut or red-cocoa invert. Microconidia are various, unicellular, round to pyriform and found in grape like bunches. Winding hyphae are frequently present. Macroconidia are multiseptate, club-formed and frequently truant. Lab tests: hair puncturing test positive, urease positive, development at 37 °C. Disease is commonly found on the feet, hands, or crotch, however can likewise be connected with provocative sores of the scalp, nails, and facial hair.

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Perforations Hair Perforation Test Trichophyton mentagrophytes , Hair puncturing test is certain. Trichophyton rubrum , Hair aperture test is negative.

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Trichophyton tonsurans Colony development is moderate, softened cowhide jump at the chance to fine, white, beige, light yellow to sulfur yellow at first glance with a yellow to dim chestnut turn around. Microconidia are various, shifting fit as a fiddle and size (pyriform, club-molded to expand formed). Macroconidia are uncommon. At the point when give they are crooked smooth dividers. Lab tests: hair aperture test commonly negative, urease positive, development at 37 °C. Development is upgraded on thiamine. Contaminations are fundamentally of the scalp. Sometimes the glabrous skin or nails are contaminated.

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Trichophyton verrucosum Colony development is moderate, glabrous to delicately fleece, white, some of the time yellow or dim at first glance with no trademark shade on the turn around. Microconidia are club-formed, however are uncommon or missing. Ordinarily, chlamydospores in chains are seen. Macroconidia have a "rodent tail" appearance, yet are infrequently observed. Lab tests: hair puncturing test negative, urease negative, development at 37°C. Development is improved on media with thiamine and inositol, and is more fast at 37 ºC than at 25 ºC. Contamination is more regular on cows or other ranch creatures. Contamination in people is commonly found on the scalp, facial hair or glabrous skin.

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Trichophyton terrestre Colony development is quick, fine to smooth, white to cream at first glance with a pale, marginally yellow switch. Sporadically, disconnects may have a pink, red-cocoa, or wine-shaded switch. Microconidia are various, club-formed, with a squared-off kilter, frequently borne on short pedicels. Macroconidia are 2-8 celled and for the most part borne at right edges to the hyphae. Lab tests: hair aperture test positive, urease positive and won't develop at 37 °C. This is a geophilic growth, exceptionally basic in soil. It can likewise be separated from the hide of little well evolved creatures.

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Epidermophyton floccosum Colony development is moderate, fine, with a yellow to khaki surface shading and chamois to chestnut invert. Macroconidia are club molded, with thin smooth dividers and can be singular or gathered in bunches. Chlamydospores are frequently delivered in extensive numbers. Microconidia are truant. Lab tests: hair aperture test negative, urease positive, development at 37 °C. Contaminations are normally cutaneous, particularly of the crotch or feet.

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Microsporum canis Colony development is quick, fleece to wooly, cream to yellow at first glance with a yellow to yellow-orange switch. Microconidia are club-molded however normally are missing. Macroconidia are fusoid, verrucose, and thick walled. They have a recurved zenith and contain 5-15 cells. Lab tests: hair aperture test positive and urease positive. Disease in people happens on the scalp and glabrous skin. It is likewise a reason for ringworm in felines and pooches.

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Microsporum gypseum Colony development is fast, wool, getting to be fine to granular, cream, brownish buff, or pale cinnamon at first glance with a beige to red-cocoa invert. Microconidia are modestly inexhaustible and club-molded. Macroconidia are bounteous, ellipsoidal to fusiform, once in a while verrucose, and thin walled. They regularly contain 3-6 cells. Lab tests: hair aperture test positive and urease positive. Contamination in people is found on the scalp and glabrous skin; it is all the more as often as possible secluded from the dirt and from the hide of little rodents.

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Microsporum nanum Colony development is quick, fleece to fine, white to buff at first glance, with a red-chestnut invert. Microconidia, if exhibit, happen in little numbers. Macroconidia are various, 1-3 celled, and have a trademark pear or egg shape. Regularly macroconidia are 2 celled. Conidia are singular on the closures of short conidiophores. Lab tests: hair puncturing test positive and urease positive. Disease is seldom transmitted to people; it is the chief reason for tinea in pigs.

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Dermatophyte Differentiation Table:

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Test Your Knowledge Each obscure slide has the accompanying route catches to help you: View dermatophyte separation table View file slide Return to beforehand saw slide View revise Answer

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Unknown 1 Colony development is quick, fleece to wooly, cream to yellow at first glance with a yellow to yellow-orange invert. Reply

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Unknown 2 Colony development is decently quick, fine to granular, white to cream hued at first glance with a yellowish, cocoa or red-chestnut invert. Reply

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Unknown 3 Colony development is quick, wool to fine, white to buff at first glance, with a red-chestnut turn around. Reply

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Unknown 4 Colony development is moderate, glabrous to gently wool, white, here and there yellow or dim at first glance with no trademark shade on the turn around. Development is improved on media with thiamine and inositol, and is more fast at 37 ºC than at 25 ºC. Reply

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Unknown 5 Colony development is ease back to direct, fleece, white at first glance with a red to chestnut turn around. Reply

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Unknown 6 Colony development is fast, wool, getting to be fine to granular, cream, brownish buff, or pale cinnamon at first glance with a beige to red-cocoa turn around. Reply

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Glossary Anthropophilic A organism (dermatophyte) which becomes specially on people, as opposed to on creatures or in soil. Clavate Club-molded. Conidium A unicellular or multicellular contagious component which serves as an agamic regenerative structure. Dermatophyte A form having a place with the genera: Epidermophyton, Microsporum, Trichophyton; ordinarily infecting skin, hair and nails. Fusoid Spindle molded; ellipsoidal with two decreased finishes. Glabrous Smooth, lacking hairs. Geophilic A organism (dermatophyte) which becomes specially on substrates found in the dirt, instead of on creatures or people. Macroconidia The bigger of two sorts of conidia delivered by a similar parasite. May be multicellular. Microconidia The littler of two sorts of conidia created by a similar growth. Ordinarily unicellular. Onychomycosis Fungal contamination of the nails. Winding hyphae Hyphae bended into a winding. Commonly found in Trichophyton mentagrophytes, yet might be seen in other dermatophytes too Verrucose Having numerous warts Zoophilic A growth (dermatophyte) which becomes specially on creatures, instead of on people or in soil.

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Bibliography de Hoog, G.S., Guarro, J., Figueras, Gene & M.J. 2000. Chart book of Clinical Fungi, 2 nd ed. Centraalbureau voor Schimmelcultures. Utrecht, The Netherlands. Benecke, E.S., and Rogers, A.L. 1996. Medicinal Mycology and Human Mycoses . Star Publishing Company, Belmont, California. Kane, Julius, Summerbell, Richard, Sigler, Lynn, Krajden, Sigmund, and Land, Geoffrey. 1997. Lab Handbook of Dermatophytes. Star Publishing Co., Belmont, CA. Larone, Davise H. 1995. Therapeutically Important Fungi, A Guide to Identification , 3 rd ed., ASM Press, Washington, D.C. McGinnis, M.R. 1980. Research center Handbook of Medical Mycology , Academic Press, New York, New York. McGinnis, M.R., D'Amato, RF., Land, GA. 1982. Pictorial Handbook of Medically Important Fungi and Aerobic Actinomycetes . Praeger Publishing. Murray, P.R., Brown, E.J., Pfallen, M.A., Tenover, F.C., Yolken, R.H., Manual of Clinical Microbiology , 7 th Edition, ASM Press, Washington, D.C. Rebell, Gerbert, Taplin, David. 1974. Dermatophytes, Their Recognition and Identification. College of Miami Press, Coral Gables, Florida. Rippon, J.W., 1974. Therapeutic Mycology The Pathogenic Fungi and The Pathogenic Actinomy

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