Clinical Applications
Slide 2Testing Categories Molecular Diagnostic Labs Infectious illnesses Bacterial, viral qualities Genetic testing Autosomal prevailing/latent Heterozygous, Homozygous Wild-sort versus Mutant versus Transporter Pharmacogenomics or pharmacogenetics Subsection of hereditary testing Genes influencing drug digestion
Slide 3Infectious Diseases MRSA VRE Group A Strep Group B Strep TB HIV HCV CMV Flu Stop me when you're exhausted…
Slide 4Autosomal Dominant Pp Achondroplasia Antithrombin inadequacy Cleft Chin Familial hypercholesterolemia Facioscapulohumeral solid dystrophy Hereditary spherocytosis Huntington's Disease Hypertrophic cardiomyopathy Malignant hyperthermia Marfan Syndrome Neurofibromatosis Pfeiffer disorder Polycystic Kidney Disease
Slide 5Autosomal Recessive Cystic fibrosis Tay-Sachs sickness Chronic granulomatous malady Thalassemia Alpha 1-antitrypsin lack Haemochromatosis sorts 1-3 Wilson's illness Homocystinuria Galactosemia Glucose-6-phosphate dehydrogenase insufficiency Phenylketonuria Albinism Certain types of Spinal strong decay Xeroderma pigmentosum pp
Slide 6Pharmacogenetics Warfarin digestion Polymorphisms VKORC1 Vitamin K epoxide reductase complex 1 CYP2C9 Part of cytochrome P450 family FDA testing suggested
Slide 7Applications of PCR DNA? RNA? PCR? Pervasive PCR, qRT-PCR, RT-PCR Current utility: High level affectability/specificity Rapid, particular microbial identification Some growths Genetic illnesses Test improvement/look into constraining variables
Slide 8Example: MRSA Coagulase positive Staphylococcus sp. Case affirmed ICU by means of standard strategies 2 cases affirmed on different wards before How might PCR be valuable? Fast affirmation of MRSA on ICU pt Investigate hereditary associations Environmental testing for disease control
Slide 9Example: Bcl-2 Translocation of Bcl-2 quality 18 to 14 at Ig substantial chain locus Overproduction of Bcl protein Apoptosis is upset prompting to neoplasia Follicular lymphoma PCR application Use 2 distinct preliminaries to get t(14:18) Bcl-2 and JH (overwhelming chain)
Slide 10Example: Bcl-2
Slide 11Example: Bioterrorism Mysterious white powder PCR application Use groundworks/tests - R/O pernicious episode Check for B. anthracis destructiveness elements LF, PA, EF, and CAP (poly-D-glutamyl case through pXO2)
Slide 12Virtual Tour
Slide 13Test Menu HPV (high hazard) HIV stack HCV qual. CMV B. henselae M. tuberculosis CF screen Sex assurance HEMONC TCR Gamma revamp BCR/ABL qual./quant. BCL-2 translocation FVL (FV R506Q) PT G20210A
Slide 14Clean Room
Slide 15Clean (cont.)
Slide 16Clean (cont.)
Slide 17Pre-Room
Slide 18Pre-Room (cont.)
Slide 19Pre-Room (cont.)
Slide 20Pre-Room (cont.)
Slide 21Post-Room
Slide 22Post-Room (cont.)
Slide 23Post-Room (cont.)
Slide 24Post-Room (cont.)
Slide 25Post-Room (cont.)
Slide 26Post-Room (cont.)
Slide 27Post-Room (cont.)
Slide 28Digene Hybrid Capture II Technology HPV (high hazard) Combination of advancements Classic sandwich Denature DNA Hybridize to RNA test Capture half and halves React w/labeled Abs Add Chemiluminescent substrate Detect flag
Slide 30Summary History and nuts and bolts of atomic science Various Molecular Techniques Clinical applications
Slide 31Multimedia Refresher https://kx.afms.mil/labreadinessskills
Slide 32Multimedia Refresher
Slide 34Questions
Slide 35Graphics References http://www.linnaeus.uu.se http://www.vetmed.uni-giessen.de http://www.devicelink.com http://www.people.virginia.edu http://www.msu.edu http://www.mie.utoronto.ca http://www.concord.org http://www.rothamsted.bbsrc.ac.uk http://www.carkanmoil.com http://www1.qiagen.com
Slide 36Graphics References (cont.) http://textbookofbacteriology.net http://www.vet.ed.ac.uk http://nitro.biosci.arizona.edu http://wikipedia.org http://www.blobs.org http://www8.georgetown.edu http://www.offthemarkcartoons.com http://www.cartoonstock.com http://depts.washington.edu http://www.cdphe.state.co.us http://content.answers.com
Slide 37Graphics References (cont.) http://www.edisonticker.com/http://www.lionden.com/http://www.dadamo.com/wiki/http://distancelearning.ksi.edu/demo/bio378/http://www.cnx.org/http://www.bact.wisc.edu/http://kconline.kaskaskia.edu/bcambron/http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/http://kvhs.nbed.nb.ca/heroic/science/http://biochem.ncsu.edu/personnel/maxwell/
Slide 38Graphics References (cont.) http://ghs.gresham.k12.or.us/science http://fig.cox.miami.edu/~cmallery/255/255chem/http://maxiangru.blogspot.com/http://www.thetutorstudio.com/http://www.sciencecartoonsplus.com/Genes VIII, Pearson/Prentice Hall http://www.ergito.com Concepts of Genetics, Pearson/Prentice Hall
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