Biosafety in Biomedical Research facilities

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Biosafety in Biomedical Laboratories Marshall University

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Major Safety Concerns Safe Handling & Containment of Infectious Agents Recombinant DNA (rDNA) OSHA Bloodborne Pathogens Standard Management of Biohazard Waste Material Other concerns not tended to in this presentation: Human Subject Protection (ORI, Dr. Winger) Radioisotope Use (RSO, Dr. McCumbee) Use of Animals in Research (Dir. ARF, Dr. Howard) Chemical (Safety & Health, Nathan Douglas)

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Chemical & Biological Safety Officer Nathan Douglas Marshall University Safety & Health Sorrell Maintenance Building, 215 1 John Marshall Drive Huntington, WV 25755 (304) 696-3461 (304) 208-7385 cell

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General Principles of Biosafety Purpose of Containment: To shield lab staff from introduction to perilous organic operators Bacteria, infections, growths, parasites Recombinant DNA Potentially risky cell lines Transgenic creatures or plants Biological poisons To prepare for the arrival of these biohazard materials

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General Principles of Biosafety Three components of regulation: Facility outline (Engineering) Lab practice and methods (Administrative) Personal Protective Equipment (PPE) Extent of control relies on upon Level of hazard Nature of organic specialist

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Primary Barriers - Safety Equipment Biological Safety Cabinet (Laminar Flow Hood) secures you and the materials you are working with Chemical Fume Hood Centrifuge Safety Canister Sharps compartment & Broken glass box Biohazard squander holder Personal Protective Equipment gloves, laboratory garments, wellbeing glasses, goggles, confront shield, outfits, shoe covers

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Biological Safety Cabinets (BSC) Class I and II BSCs give a powerful regulation framework to safe treatment of direct to high hazard microorganisms Biosafety Level (BSL) 2 and 3 specialists Class II BSCs ensure investigate material through high proficiency particulate air (HEPA) filtration. The Biotech building has imparted offices to Class II, Type A2 cupboards Not reasonable for use with radionuclides or harmful chemicals 70% air distribution & fumes to room

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Biological Safety Cabinet Class II, Type 2A

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Secondary Barriers - Facility Design and Construction Security measures and physical division of lab work territories from regions of free Swipe card access to explore passages Key access to research suites Break zones outside of research ranges Availability of disinfecting stations Handwashing and eye washing offices Emergency eyewash and shower stations in corridor Autoclave (just Room 119 for decon) UV lights in Biosafety Cabinets – Use Caution Separate ventilation frameworks

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Four Biosafety Levels (BSLs) BSL1-4 speak to conditions under which the operator can be securely taken care of. Mixes of lab offices lab rehearses procedures wellbeing hardware Selected in light of operations performed and courses of transmission of irresistible operators and additionally rDNA

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Biosafety Levels 1-2 BSL 1: suitable for work with living beings known to not bring about disease in solid grown-up people E. coli, Bacillus spp. Excluded classes of rDNA work BSL 2: indigenous, direct hazard operators Hepatitis B, HIV, some Salmonellae Human inferred blood and blood items Cell culture work and some rDNA Primary Hazard: Skin break, mucous film introduction or ingestion

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Biosafety Levels 3-4 (none in BBSC) BSL 3: indigenous or intriguing specialists with potential for respiratory transmission or deadly results M. tuberculosis, St. Louis encephalitis infection (CNS inconveniences and demise) Primary and Secondary boundaries to ensure work force in bordering regions BSL 4: deadly intriguing operators where there is no antibody or treatment Ebola and Marburg infections Separate office or HVAC disengaged zone

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Which Biosafety Level To Use Ask your lab head or essential specialist Read Centers for Disease Control's (CDC) " Biosafety in Microbiological and Biomedical Laboratories " Go to American Biological Safety Association site for rundown of irresistible operators and their BSL Read National Institutes of Health's (NIH) "Rules for Research Involving Recombinant DNA Molecules" Contact Dr. Primerano b y calling 304-696-7338 or messaging All assets accessible on Marshall's Institutional Biosafety Committee site page:

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Biosafety Level 1 Practices: Known as "Standard Microbiological Practices" Access to research facility is constrained or limited when trials are in advance Persons wash hands in the wake of taking care of reasonable materials, in the wake of expelling gloves, and before leaving lab Eating, drinking, smoking, taking care of contact focal points, and applying makeup are not allowed in the work regions. Nourishment is put away outside the work zone Mouth pipetting is precluded; mechanical pipetting gadgets are utilized

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Biosafety Level 1 Practices: Known as "Standard Microbiological Practices" Policies for the protected treatment of sharps are taken after All methods performed precisely to minimize the production of sprinkles or mist concentrates Work surfaces sterilized on finishing of work or at end of the day, and after any spill or sprinkle of reasonable material with disinfectant All societies, stocks, and other controlled squanders are purified before transfer. We utilize autoclave A creepy crawly and rat control program is as a result Biohazard signage on external lab entryways

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Biosafety Level 2 Practices: Includes all practices from BSL-1, or more: Only people who've been educated regarding the potential dangers and finished vital inoculations may enter the Lab staff get preparing on potential risks connected with the work, insurances to counteract exposures, and presentation assessment systems Lab coats and eye/confront assurance are worn in the lab and are not worn outside of lab ranges. Gloves are expelled before leaving lab Extreme alert utilized with polluted sharps to dodge autoinoculation and airborne. Plasticware ought to be substituted for china when conceivable. Broken glass is not took care of by hand, must be evacuated mechanically

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General Biosafety Advice Discuss particular security issues with your essential agent or lab head Dr. Primerano is constantly accessible for inquiries at 304-696-7338 and Labs ought to have composed security conventions that are lab/explore particular Read " Biosafety in Microbiological and Biomedical Laboratories " as required Review Animal Biosafety Levels when research includes irresistible ailment work with test creatures

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General Biosafety Advice Use a scientist's jacket and proper wellbeing hardware for all strategies Don't take scientist's jacket home to wash it. Utilizing permanent ink, name the coat's tag with your initials and lab number Place the coat in a marked biohazard pack and offer it to Julia Schreiber in room 119 or 121 Don't wear open-toed shoes or shoes Keep hair short or tied back If you have glove hypersensitivities, vinyl or nitrile gloves may help

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Work with Cell Lines and Cultures Consider all cell lines and societies irresistible All work ought to be done in an organic wellbeing bureau Appropriate biosafety rehearses for taking care of cells known or suspected to contain a pathogenic infection or microbes must be utilized when working with the cell culture All essential and changeless cell lines must be taken care of utilizing BSL-2 practices and control Any examination including changed or tumor cell lines falls under BSL-2 hones

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Work with Cell Lines and Cultures BSL-1 practices and regulation might be utilized for cell lines that meet the greater part of the accompanying criteria. Cells must be: non-primate non-human affirmed not to contain a primate infection, pathogenic microscopic organisms, mycoplasma or parasites entrenched

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Work with Cell Lines and Cultures Waste arrangements gathered legitimately All waste arrangements and unused cells must be disinfected for 24 hours before autoclaved 10% dye arrangement, at conclusive fixation fade with 5.25% accessible hypochlorite

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rDNA Guidelines National Institutes of Health (NIH)

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Recombinant DNA (rDNA) As characterized by the NIH rules rDNA atoms are either: DNA particles developed outside cells by joining characteristic or manufactured DNA fragments to DNA atoms that can recreate in a living cell, OR Molecules that outcome from the replication of those portrayed above

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rDNA Guidelines BSL-1 is fitting for most rDNA work at Marshall (yet not all) Principal examiners must be acquainted with the rules Each lab must have Institutional Biosafety Committee endorsed conventions for utilization of rDNA Consult essential specialist or lab head with inquiries Review the rDNA rules as important

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Bloodborne Pathogens Standard OSHA

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Bloodborne Pathogen Standard A duplicate of the OSHA Bloodborne Pathogen Standard is accessible on the organization's site Additionally, connections to the BBP Standard are accessible on the accompanying Marshall University sites:

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Bloodborne Pathogen Standard Bloodborne pathogens are Pathogenic microorganisms introduce in human blood and can bring about sickness in people Includes, however are not restricted to hepatitis B infection (HBV) and human immunodeficiency infection (HIV ) Others include: hepatitis C, intestinal sickness, syphilis, babesiosis , brucellosis, leptospirosis , arboviral contaminations, backsliding fever, Creutzfeld-Jakob Disease, Human T-lymphotrophic Virus Type 1, and viral hemorrhagic fever

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Bloodborne Pathogens The accompanying substances are particularly secured under the standard: Human blood,